No story can truly come to life without a series of characters at its heart.
| William Pateron | Protagonist |
| William Dampier | Pirate who crossed the Isthum with Lionel Wafer. Later a travel writer of “A New Voyage Around The World”. |
| Lionel Wafer | Buccaneer |
| Captain Richard Long | English Captain. Sailed the Rupert to the Isthmus on 05 Dec 1697. Spy for the English |
| Captain Robert Pennecuik | Argued with everyone. Died at see after deserting the colony. |
| Walter Herries | Surgeon |
| Captain Robert Pincarton | Captured by the Spanish whilst aboard the Dolphin |
| Captain Robert Drummond | |
| Captain Thomas Drummond | Brother Robert. Imprisioned by James Byres. |
| Tomas Fullarton | |
| Captain Robert Allison | Paterson’s friend. Captain. Piloted the first expedition from Crab Island to Darien |
| Captain Ambrosio | Tull leader on Darien. Ally of the scots |
| Andreas | Tull leader. Ally of the scots |
| Earl / Duke Archibald Campbell Argyll | 10th Earl, later Duke of Clan Campbell. |
| James Balfour | |
| Baron John Hamilton Belhaven | 2nd Baron |
| Earl Richard Coote Bellamont | 1st Earl of Governor of New York. Sympathetic to the scots but adhered to the English proclamation. |
| Sir Robert Blackwood | Joint Founder |
| Reverend Francis Borland | Minister on 2nd Expedition |
| James Byres | Councillor. Condemned by directors for treachery. Imprisons Thomas Drummond at Darien. |
| Colonel Alexander Campbell | … of Fonab. Won a victory over the Spanish at Toubascanti |
| Captain Colin Campbell | |
| James Campbell | Merchant in London |
| President Conde de Canillas | President of Panama |
| Don Luis Carrizoli | Commanded the Spanish malitia at Toubascanti |
| James Chiesly | Joint Founder |
| Sir Robert Chiesly | |
| Major James Cunningham | Councillor of the first Colony which he deserted. |
| Captain Diego | Tull leader. Ally of the scots |
| Colonel John Erskine | …of Carnock |
| Andrew Fletcher of Saltoun | Friend of Paterson |
| Captain Thomas Green | Hung on Leith sands falsely accused of piracy. |
| Don Melchor de Guevara | Led the first attack on the peninsula. |
| John Haldane | …of Gleneagles. Discovered Smyth’s embezzlement of the Company’s money |
| Lord Basil Hamilton | |
| Duke James Douglas Hamilton | |
| James Hodges | Pamphleteer – likely hired by Hamilton to respond to Herries book. |
| Captain Robert Jolly | Argued with Pennecuik and was arrested in Darien. |
| Major John Linsday | |
| Patrick MacDowall | Friend of Paterson. Supercargo. |
| Roderick MacKenzie | Responsible for the arrest of Thomas Green on the charge of piracy. Secretary of the Company |
| Captain Lachlan MacLean | |
| Sir Patrick Hume | …of Polwarth. 1st Earl of Marchmont. Opponent to the Company |
| Captain James Montgomerie | Quarrelled with Pennecuik, deserted Darien with Joly. Censured by the Company |
| Richard Moon | Friend of Paterson. Brought provisions to the first colony |
| Doctor John Munro of Coul | Accused of embezzlement by the Company |
| William Murdoch | Sided with Jolly |
| Lieutenant-General John Nanfan | L-G of New York |
| Roger Oswald | Letters contain a vivid account of Darien. |
| James Maule | Earl of Panmure |
| Henry Paton | |
| Captain Pedro | Tull leader. Ally of the scots |
| Don Juan Pimienta | Governor of Cartegena. Organised the second attack on the colony. Accepted their surrender. |
| Hugh Rose | |
| Sir Paul Rycaut | English resident at Hamburg. Successfully prevented the Scots opening a subscription book there. Spied on their shipping. |
| Captain Edward Sands | Moon’s colleague. Jamaican shipmaster. |
| James Smyth | Embezzler. Huge story. Paterson’s friend. |
| Reverend Alexander Shields | |
| Interpreter Benjamin Spence | Captured by the Spanish |
| Reverend Alexander Stobo | |
| John Hay | 4th Earl and 1st Marquis of Tweedale. Lord Chancellor of Scotland |
| John Hay | 2nd Marquis of Tweedale |
| John Murray | Earl of Tullibardine |
| Lieutenant Robert Turnbull | |
| James Vernon | Secretary of State |
| Captain Samuel Vetch | |
| Captain William Vetch | Brother Samuel |
| 2nd Duke of Queensbury James Douglas | Kings Commissioner to the Estates, 1700 |